LocoRoco creator Tsutomu Kouno dropped a few sequel hints at the Nordic Game Conference. While saying he was going to get "scolded" if he offered too much info, Kouno promised LocoRoco 2 would have:

...new gameplay based on physical calculation and crowd AI as well as a few surprises.

I just love the idea of some Sony suit telling off Kouno for divulging his plans. Brian Ashcraft

Kouno Drops Hints [Next-Gen]