If you're here strictly for girls in lingerie cavorting with plastic instruments, that's at the four minute mark. No one's naked, so that's why I didn't tag this NSFW.
Otherwise, Playmates Karissa & Kristina Shannon give a brief interview about their love of Guitar Hero, as does Playboy founder Hugh Hefner.
"The game is very popular, it's youth oriented. It's a good Playboy connection," says Hef.
Guitar Hero 5: Behind the Scenes of Playboy Playmates' Risky Business Video @ The Playboy Mansion [YouTube user elmundotech]