Sadly, I don't think the trailer is a puzzle like the first two teaser trailers we saw back in March. However, the animation looks pretty spiffy and Layton fans are sure to love all the new plot teasers in this 93 second long cinematic treat. If they can understand Japanese, that is.
Professor Layton and the Eternal Diva comes out in Japan on December 19. The plot's completely separate from the video game series. However, the film is being produced by Pokémon film veteran Masakazu Kubo, so we can expect fidelity to character design if nothing else.